The European Commission had found that Google's shopping service had seen traffic jump 45-fold in the United Kingdom when it began to abuse its dominance.
Google confirmed the appeal to Business Insider but declined to comment further or give any detail.
The Silicon Valley giant had responded to the fine at the time of its announcement by saying that it "respectfully" disagreed with the legal argument being pursued.
Klopp laments heavy defeat, fault Mane's Red Card
Of course, it was going to end up this way with the visitors forced to play the second half down to 10 men for about 53 minutes. The game's major talking point came when Mane chased a long ball over the top and raised his boot as Ederson came rushing out.
Google had been given 90 days to stop the favouritism or face a penalty of up to 5% of the average daily turnover of its parent company Alphabet.
It is anticipated to take years before the Luxembourg-based General Court rules on the case.
Brussels accused Google of giving its own service too much priority in search results at the expense of other price comparison services, such as TripAdvisor and Expedia. Google has previously said it disagreed with the European Union decision and had indicated it would file an appeal. "And most importantly, it denied European consumers a genuine choice of services and the full benefits of innovation". Last week, the EU's highest court backed Intel Corp.'s appeal of a €1.06 billion antitrust fine from 2009, seen as dealing a blow to the antitrust regulator.
While the Google case is not the same as that of Intel, the judgment has been welcomed by companies under European regulatory scrutiny because it raises the bar for the regulator to prove wrongdoing. The first relates to Android, its operating system on a smartphone, and the second AdSense, its advertising network.